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A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies Download PDF.The amount of light generated is proportional to the activity of luciferase. E152A: 10 × 10ml: Reporter Lysis 5X Buffer. Add one volume of 100X Luciferase Substrate to 100 volumes of Assay Buffer and mix well. 90,91 When the activated protease digests its substrate sequence, the luciferase is opened, allowed to adopt its native confirmation, and recovers its activity. Luciferase-based reporter gene assays are widely used due to their ultrasensitive detection capacity and wide dynamic range. Such applications include the luciferase assays for gene expression and other cell-based assays. Note: The reconstituted luciferase assay solution is not stable. Ultra-Glo™ rLuciferase Luciferase Assay Substrate. Add 100 µl of ONE-StepTM Luciferase D-Luciferin, notably Firefly luciferin (also beetle luciferin), is a bioluminescent substrate for the enzyme luciferase. The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level.
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Read the signal immediately using a luminometer. Luminescence assays, on the other hand, are different because no light is directed at the sample.The firefly luciferase (PDB ID: 1BA3), found in the North American species Photinus pyralis, catalyzes the production of yellow-green light via oxidation of firefly luciferin in the presence of MgATP and molecular oxygen 1. 238000003670 luciferase enzyme activity assay Methods 0.
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Luciferase Cell Culture Lysis Buffer (5X) Severely irritating to eyes.